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Image Search Results
Journal: Oncotarget
Article Title: The anti-apoptotic BAG3 protein is involved in BRAF inhibitor resistance in melanoma cells
doi: 10.18632/oncotarget.18902
Figure Lengend Snippet: Representative images of BAG3 negative (score 0), BAG3 low positive (score 1) and BAG3 high positive (score 2) metastatic melanoma samples stained using a monoclonal anti-BAG3 antibody revealed by using a biotinylated secondary antibody. Sections were counterstained with hematoxylin. ( A ) mut, mutation; WT, wild type. Fisher exact test was calculated by using 2 × 3 contingency tables. ( B , C ) A375 extracts were immunoprecipitated with an anti-BAG3 monoclonal antibody and immune complexes were then immunoblotted with antibodies recognizing BRAF, BAG3, Hsp70, or GAPDH as indicated. Immunoprecipitation with mouse IgGs was used as negative control ( D ) BAG3 down-modulation reduces levels of BRAF protein and affects ERK phosphorylation in A375 cells. A375 cells were transfected twice consecutively with a BAG3-specific or a non-targeting (NT) siRNA (200 nM), with the second transfection time being 72 hrs after the first one. After 120 hrs collected from the first transfection cells were analysed by western blot using anti-BAG3 polyclonal, anti-BRAF, anti-pERK and anti-ERK1 antibodies. Anti-GAPDH antibody was used as loading control. The levels of BAG3, BRAF were quantified by densitometry and normalized to GAPDH (O.D. BAG3/O.D. GAPDH; O.D. BRAF/O.D. GAPDH) ( E ) * p < 0.05 > 0.01; ** p < 0.01 > 0.001.
Article Snippet: Anti-BAG3 rabbit polyclonal and
Techniques: Staining, Mutagenesis, Immunoprecipitation, Negative Control, Phospho-proteomics, Transfection, Western Blot, Control